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Maintaining a laboratory notebook helps students hone their record-keeping and meet the ASM curriculum guidelines for scientific thinking and laboratory skills 1d, “Document and communicate the methods, results, and conclusions.” However, grading a laboratory notebook is time-consuming for the instructor and, for hand-written lab notebooks, there is an additional challenge of reading student handwriting. The lab notebook quiz provides a quick, easy-to-grade solution. A laboratory notebook quiz has been previously described as one part of the lab notebook assessment for an upper-division microbiology course (Fleming, 2023). Here, we describe its use as the sole means of assessment of the lab notebook in a non-majors lab with 8 sections of up to 24 students each. We discuss specific learning objectives that can be assessed using the quiz, as well as considerations for working with Teaching Assistants and reducing the possibility of cheating between sections held at different times of day.