Welcome to ASMCUE in Seattle, Washington! Registered attendees are able to download the mobile app onto their phone, create a log-in, and make a personalized schedule and chat with attendees. Questions? Email [email protected]
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“Glow Up” (BIOL 3090) reimagined a standard pGLO transformation lab into a collaborative undergraduate research experience at UNT Dallas. Although the pGLO transformation system is widely used, it is almost always qualitative. This CURE redesigned pGLO as a quantitative research platform in which transformation efficiency, measured as GFP-positive colony counts per microgram of plasmid DNA, was used to assess bacterial response to hydrogen peroxide-induced oxidative stress. Each student selected and investigated a different experimental variable (essential oils or antioxidant controls), performed quantitative analysis and comparative interpretation across conditions, and contributed individual data to a collective dataset. Preliminary findings revealed differential antioxidant responses across treatments, including reproducible rescue effects under oxidative stress. Students shared these findings through presentations at an institutional research symposium, demonstrating scientific communication as a course outcome. Attendees will gain practical strategies for redesigning traditional transformation labs as quantitative CUREs without requiring specialized instrumentation.
