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At Universidad Nacional de Rosario (UNR), Argentina, Microbial Physiology and Genetics are taught as independent courses in consecutive semesters, although both explain the same biological processes from complementary perspectives. In this Microbrew, we propose an integrated teaching strategy for undergraduate Biotechnology students in which laboratory activities from both courses are linked through common biological questions. In the first module, students will compare UV sensitivity in recA⁺ and recA⁻ strains, and then investigate DNA damage repair by monitoring SOS induction with a transcriptional reporter fusion. In the second, they will analyze the physiological consequences of defective lipoic acid biosynthesis—auxotrophy and impaired sporulation—and then complement lipM mutants through natural transformation of Bacillus subtilis. This framework could be extended to additional topics, including light-regulated motility, two-component signaling, and biofilm formation. During this Microbrew, participants will discuss this approach and help refine the activities before classroom implementation.
